FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20600136
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT944550.4585163903772286No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG461460.22400822984857965No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG284310.13801365194870557No Hit
CTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACAT282580.1371738516677754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATGGC285000.042.33604470
TCACCCG200800.039.3081363
CGGACAC199700.039.13712313
TAGCATA96950.038.927959
ACCCGGC207200.037.659555
ACACGGA205750.037.51431716
CCGGACA210550.037.3175812
ACGGACA210300.037.0966718
CCAGTAG100100.036.9322285
CACCCGG212650.036.82354
CCCGGAC213900.036.58690311
CGGACAG214400.036.32148419
GCCCGGA219350.035.6143410
CACGGAC220800.035.3181417
GGACACG223350.034.8682314
GACACGG225100.034.427315
CTCACCC241900.033.1261832
GTAGCAT114300.032.805078
CAGTAGC114950.032.6449856
CCTCACC253350.032.3359871
Produced by FastQC (version 0.11.5)