FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13667771
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGAT38050.025.36653148
TAGCTCT40050.023.91624540
CTCGATT40000.023.87074349
ATTCCGT40350.023.58973553
TTCTCGA42850.022.44126547
CGGACAC44750.022.17834913
ACCCGGC46300.021.4289725
GCTACCG28100.021.3569570
TTCCGTG44450.021.18164654
ACACGGA48050.020.51110816
GCATGGC77150.020.355670
ACGGACA48400.020.29063618
TAGCATA14350.019.5866019
GGTGCAT49600.019.55008367
CGGACAG50450.019.32786419
ATAGCTC50050.019.20655339
TGATAGC52050.019.1345137
CACCCGG51650.019.074784
CCGGACA51600.018.96484412
TTGATAG52650.018.85026736
Produced by FastQC (version 0.11.5)