FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005294030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005294030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17821092
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG2110291.1841530249661467Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCGGTTTGAGTGAGACGAGACGAGACGCGCCCCTCCCACGCGGGGAAGG309890.17388945638123635No Hit
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGT250710.14068161479666902TruSeq Adapter, Index 9 (97% over 37bp)
GATCAATCGCCCCCGGGGGTGCCTCCGGGCTCCTCGGGGTGCGCGGCTGG192200.10784973221618517No Hit
GGGGGGGCCTGGTGGGGTTGCGCGCACGCGCGCACCGGCCGGGCCCCCGC192130.10781045291725108No Hit
CGAGACGAGACGCGCCCCTCCCACGCGGGGAAGGGCGCCCGCCTGCTCTC183570.10300715579045325No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)