FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005294031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005294031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17821092
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG2231631.25224088400419Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG258890.14527168144353894No Hit
GGCAGAGACAGAGGCGGCGGCCCGGGGGATCCGGTACCCCCAAGGCACGC214110.12014415278255677No Hit
GGGGGCCGCCCGCAAGTCGACAACCACTGGAGGCGACAGCGAGGGCTGTC184370.10345606206398575No Hit
GCGGGAACGACACACCACCGTTCGGCCTCGGGCACCTGAGGGACAACCCG181940.10209250925813076No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)