FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005294032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005294032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6670849
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG3138044.704108877295829Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTACAGT149010.2233748657779542TruSeq Adapter, Index 23 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTACAGTGCA92250.13828824486958108TruSeq Adapter, Index 23 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTACAGTGTTT91840.13767363044793848TruSeq Adapter, Index 23 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTACAGTGCTA91610.13732884674799264TruSeq Adapter, Index 23 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTACAGTGCTC88320.13239694077920217TruSeq Adapter, Index 23 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTACAGTGCTT87270.13082292823597116TruSeq Adapter, Index 23 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTACAGTGCAT86650.12989351130568239TruSeq Adapter, Index 23 (97% over 37bp)
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT86470.12962368058398563No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTACAGTGCT84800.12712025111046585TruSeq Adapter, Index 23 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTACAGTGTA83330.12491663354994244TruSeq Adapter, Index 23 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTACAGTGTT81640.12238322288512303TruSeq Adapter, Index 23 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTACAGTGCAC81350.12194849561127825TruSeq Adapter, Index 23 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTACAGTGTAC80610.12083919153319164TruSeq Adapter, Index 23 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTACAGTGCAA80450.12059934200279454TruSeq Adapter, Index 23 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTACAGTGTTA79670.11943007554210865TruSeq Adapter, Index 23 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTACAGTGTGC75010.11244445796929295TruSeq Adapter, Index 23 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTACAGTGTTC74090.11106532316950961TruSeq Adapter, Index 23 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTACAGTGTGT72120.10811217582649525TruSeq Adapter, Index 23 (97% over 37bp)
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG72050.10800724165694651No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTACAGTGTCT70360.1054738309921271TruSeq Adapter, Index 23 (97% over 37bp)
CGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT69150.10365996891849898No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG67320.10091668991458208Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTACAGTGTG66730.10003224477124276TruSeq Adapter, Index 23 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)