FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005294036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005294036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3142549
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG324531.0326967057633787Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGT80350.2556841595787369TruSeq Adapter, Index 6 (97% over 36bp)
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT33990.10816060465564736No Hit
CGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT33120.10539215140320804No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)