FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005294039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005294039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3463967
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG767502.2156677589595977Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG100830.29108244968846414No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)