FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005294441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005294441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35371605
Sequences flagged as poor quality0
Sequence length5-50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTCG16350.016.95758823
CGTGCGA16800.016.20195810
CGGTATG16850.016.10361543
TTCGGTA20250.014.14175426
CGTTCGG19850.013.98055924
CGTAGCG16650.013.05949315
CGCGGTA20700.012.71913743
CCGTGCG28050.012.3366379
CGGTAAG24000.012.06212528
TCGCATA13050.011.94078440
CGAACGA17200.011.7823714
TATACCG22450.011.2795696
TCGTTGT20900.011.12715234
GCGAACT27600.010.72017628
GTATAGA72000.010.6743121
TTACTCG32600.010.62334219
TAACGGC23200.010.55887436
CGGTCGT20050.010.52380310
TACTCGC20100.010.50732920
CATCGTT23250.010.48078632
Produced by FastQC (version 0.11.5)