FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005294445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005294445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6962083
Sequences flagged as poor quality0
Sequence length5-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGA3650.021.02286110
CCGTGCG5000.017.4780469
CGAGAAT6050.014.44736614
TCGTTGT4850.014.35203934
CGTAGCG2309.567884E-1013.90358315
TCGCATA2651.2878445E-913.62967840
TATACCG4100.013.5166346
TGTCGAC950.007698199713.46200810
GCGAGAA6450.012.88934213
CCGTTCA2851.8226274E-911.9661439
CGCCGTA2709.371433E-911.93011425
TATCGAT3859.276846E-1111.82935542
CGATAAC3251.2854798E-811.67809643
CATCGTT5900.011.59300532
CCGATAG2657.815652E-811.48430
CGACGTT2607.860399E-811.47928614
CGGTCGT3351.6370905E-1011.45275210
TCGTTCG3208.58563E-1011.37729323
TTAGCGC1752.3312456E-411.25834231
TCGATTA4102.7830538E-1011.10843344
Produced by FastQC (version 0.11.5)