FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005294446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005294446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57877758
Sequences flagged as poor quality0
Sequence length5-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGA30550.020.90242810
TCGTTCG31200.016.08529723
CCGTGCG45800.015.9409919
TCGTTGT40950.015.76980834
GCGAAGT31350.015.21337232
CGTTCGG33050.015.20110424
CATCGTT44150.014.58613532
CGCGGTA33650.014.48245943
TTCGGTA35400.014.33803426
CGAGAAT49100.014.00391214
CGGTAAG36200.013.99980228
GCGAGAA52200.013.53889313
CGGTATG26150.012.84911943
CGAACGA29700.012.18486714
TATACCG38200.012.1599016
TCGCATA25300.012.10996740
CTCGCTA34500.012.09351513
CGTAGCG24650.011.74517215
CCGTTTA40250.011.476513
TAACGGC38750.011.45640836
Produced by FastQC (version 0.11.5)