FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005294447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005294447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57877758
Sequences flagged as poor quality0
Sequence length5-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGA29550.021.83193810
TCGTTCG29350.016.5948523
CCGTGCG46000.015.7370249
CGTTCGG31650.015.3364724
TCGTTGT38050.014.66869134
CGTAGCG24800.014.17363715
CGCGGTA31150.014.15837243
CGGTATG26100.014.14701843
TTCGGTA35800.013.93393226
CGAGAAT47150.013.73504414
CATCGTT40800.013.51709432
CGGTAAG37050.013.32660828
GCGAGAA51700.013.0535613
TCGCATA25850.012.78208240
GCGAAGT32300.012.17643832
TACCCGC41200.011.78099911
TTGCGAT34450.011.44183716
GCGGAAT32100.011.35221719
TATACCG39950.011.24622256
TAACGGC36650.011.22893536
Produced by FastQC (version 0.11.5)