FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005294448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005294448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15832124
Sequences flagged as poor quality0
Sequence length5-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG42850.013.6641415
TACACTG47850.011.5615335
TCTATAC33350.011.2971353
CTATACT49050.011.1045094
TTTAGCG6900.010.9475926
ATACTGT57000.010.9140836
CGTGCGA4205.456968E-1210.74293110
GTATAGA35100.09.9985141
TCGTTCG5200.09.94132723
GTGTTAG44450.09.8812831
TAGACTG39850.09.8312185
GTATTAG40300.09.8303221
TAGACAG52150.09.7826915
GTATAGG28200.09.6963791
GTCTTAG39100.09.6364331
TCTGTCG14500.09.6317158
CTAGACT35950.09.5217354
CGGTATG6500.09.48693843
GTCCTAG43250.09.4585041
GTGTAAG40000.09.4196121
Produced by FastQC (version 0.11.5)