FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005294449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005294449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15832124
Sequences flagged as poor quality0
Sequence length5-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGA4950.015.63178210
TATACTG43600.013.9017555
TACACTG44200.011.6220485
ATACTGT54800.011.1387546
CCGTGCG9400.010.9754379
CTATACT45750.010.9462764
TAGTCCG9150.010.5705715
CCTATAC27400.010.2757953
GTATAGG28450.010.0483061
TCTATAC34900.010.0382243
GTGTAGA39150.09.9922471
GTACTAG14400.09.8515591
GTATAGA35100.09.5530271
TACACAG49000.09.4308195
TAGGACC21650.09.3319314
CTACACT43450.09.2997144
CCTACAC37600.09.2602323
TACCCTG33750.09.2342425
TAATACT42700.09.1610464
TAGACTG37550.09.0438675
Produced by FastQC (version 0.11.5)