FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005294450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005294450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59463539
Sequences flagged as poor quality0
Sequence length5-50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG125150.010.1654045
GTATAGA105700.09.39891
GTGTTAG151700.08.9817131
TACACTG164950.08.9742385
TAGACTG128650.08.8382635
TAGACAG186800.08.8318215
GTGTAAG137750.08.489381
GTATTAG114150.08.3271981
GTATAGG86150.08.1195761
GTGTAGA131700.07.9669881
CTATACT124900.07.95396664
GGCTAGA150550.07.9528821
GTATAAG98050.07.94383341
CTACACT143600.07.9342394
GTCCTAG142150.07.87942841
GTGTAGG136750.07.62570521
ATACTGT164300.07.586566
GTTAGCC89250.07.54903363
GTATATA257900.07.52953771
CTAGACT123350.07.4972734
Produced by FastQC (version 0.11.5)