FastQCFastQC Report
Sat 4 May 2024
EGAF00005295949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005295949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50140647
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC4924490.9821353122946339No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT3978580.7934839771812278No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG2223420.44343663933973565No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1952530.3894106113150076No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC1720300.3430948946470515No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA1231630.24563504336112774No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT1181440.23562520044864999No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1161260.2316005216286898No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1045490.20851146974629184No Hit
TCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC931350.1857475034177361No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC880080.17552226639596413No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA861850.17188649360667405No Hit
CTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCT844300.16838633933064326No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC819140.1633684543400487No Hit
CAGCAGATCATTTCATATTGCTTCCGTGGAGTGTGGCGAGTCAGCTAAAT756350.1508456801524719No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC722120.14401888352178624No Hit
AGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGA703310.14026743611824555No Hit
GTTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCCTAAGATAGAG681120.13584188492821006No Hit
GGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTA661770.13198274047002226No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCCGTATCTCGTAT628850.12541720891635083TruSeq Adapter, Index 7 (97% over 35bp)
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC582780.11622905464303242No Hit
CGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCC523220.10435046839343735No Hit
CGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTT515750.1028606591374858No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)