FastQCFastQC Report
Thu 9 May 2024
EGAF00005295963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005295963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35471393
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1506410.42468306784568627No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC1325990.3738195452318436No Hit
CAGCAGATCATTTCATATTGCTTCCGTGGAGTGTGGCGAGTCAGCTAAAT704620.19864458100080817No Hit
GTTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCCTAAGATAGAG606400.17095466197225467No Hit
GGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTA566600.15973435269373268No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG559150.15763406867049173No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT529920.14939362544910487No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC493020.13899087639439475No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT445430.12557443120432288No Hit
CGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTT419050.11813745234082011No Hit
CGGGGCATTCCGGATAGGCCGAGAAAGTGTTGTGGGAAGAAAGTTAGATT404960.11416523732236848No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)