FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005296016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005296016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49984366
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC8020821.6046657468857362No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT6062131.2128052199361696No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG3732960.7468255174027815No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT3186460.6374913307893112No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC3062330.6126575657676643No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA2138510.42783577569034287No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1828010.36571635218900245No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT1820870.36428790554230495No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1767780.35366658446763133No Hit
TCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC1565620.31322193823564753No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC1501560.3004059309264821No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC1416640.28341661870833773No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA1380190.2761243385581804No Hit
AGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGA1213020.2426798811452365No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1122260.22452220360262248No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCATCTCGTAT1103340.22073702005142967TruSeq Adapter, Index 15 (97% over 37bp)
CGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCC1004990.20106086771211623No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC1004020.20086680703322313No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT751840.15041503177213453No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCATCTCGTA736260.14729805715651167TruSeq Adapter, Index 15 (97% over 37bp)
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG710900.14222447074751335No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT680170.13607654841515845No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG672740.1345900836273486No Hit
GGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGT626000.12523915978048017No Hit
TCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA614040.1228464116159841No Hit
CCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACC612180.12247429526264272No Hit
CGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCA603580.12075375728482782No Hit
TAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCG602150.12046766783037721No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT593630.11876313485700708No Hit
CACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCA588470.11773081207031816No Hit
TGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG577720.11558013959804952No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCATCGCGTAT574130.11486191502358958TruSeq Adapter, Index 15 (97% over 37bp)
GGAAAGAGTAGAACAAGGAGTTCGATCTGTAACTGACTGTGAACAATCAA568300.11369555032467553No Hit
GTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGT559680.11197101109574942No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA558870.11180896042574592No Hit
GTGTGGTTGGTGCGCGGACACGCACTGCCTGCGTAACTAGAGGGAGCTGA551090.11025247374349012No Hit
GGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTA544210.1088760433612382No Hit
GTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCACATGGAGC543680.10877001020679145No Hit
GGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCACAT543280.1086899851845675No Hit
TTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCG503620.10075550423106297No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)