FastQCFastQC Report
Sun 5 May 2024
EGAF00005296042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005296042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49374915
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2785440.5641407180144007No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC2496240.5055684652824213No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG1203250.2436966220600076No Hit
CTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCT1022830.20715579966061712No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1017390.20605402561199349No Hit
CAGCAGATCATTTCATATTGCTTCCGTGGAGTGTGGCGAGTCAGCTAAAT957890.19400337195517198No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC937540.18988184587254478No Hit
GTTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCCTAAGATAGAG866250.17544334000372455No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT804710.1629795210786692No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAGGAATATCTCGTAT799200.16186356979044927TruSeq Adapter, Index 2 (97% over 36bp)
GGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTA760770.1540802652521022No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA632990.1282007270290997No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA623270.1262321160451618No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA601620.12184729837003265No Hit
GTGGGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGT592240.11994754826413373No Hit
CGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTT585300.1185419762241616No Hit
CGGGGCATTCCGGATAGGCCGAGAAAGTGTTGTGGGAAGAAAGTTAGATT565230.11447715909991946No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG543440.11006398694559777No Hit
TCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC496310.10051865405742977No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)