FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005296045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005296045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47676220
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC10862092.2783035232239466No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT7301091.5313902821993857No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG4965241.0414500142838505No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT4430650.9293207389344206No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC4019530.843089070400296No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA2834040.5944347097987215No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG2423160.5082533808259129No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA2415890.5067285116143855No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT2198500.46113135647079406No Hit
TCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC2063390.4327922809316678No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC1952730.4095815482015982No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC1784710.3743396603170302No Hit
AGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGA1579800.3313601623618651No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA1572620.3298541704858313No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1549850.32507820460598597No Hit
CGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCC1190640.24973456368814476No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC1178590.24720709821374262No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG934590.1960285442092515No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT903150.18943406167686952No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT843280.17687643861027574No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG837890.17574589596238963No Hit
GTGTGGTTGGTGCGCGGACACGCACTGCCTGCGTAACTAGAGGGAGCTGA833480.1748209065232101No Hit
CGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCA813490.1706280405619405No Hit
CCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACC801330.16807750278860195No Hit
TCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA797290.16723012017311775No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA796610.16708749141605606No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT784840.16461875542985582No Hit
TAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCG784000.16444256696525017No Hit
GGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGT777900.1631631031151379No Hit
GTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGT733340.1538167245641538No Hit
GGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCACAT718960.1508005458486432No Hit
GTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCACATGGAGC717960.15059079767649364No Hit
CACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCA717490.15049221603558335No Hit
TGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG710130.14894846948856263No Hit
GTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGAC676940.14198692765491897No Hit
TGACGGATGACGCCCCCGCGCCACGCCGCTCAGCGGGATACGCTTCTTGG642790.1348240275760117No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTGAAGATCTCGTAT614310.1288503996331924TruSeq Adapter, Index 10 (97% over 36bp)
GGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGACCGAAGAC593260.12443520060944428No Hit
TTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCG593120.12440583586534334No Hit
CTGAATTGGGTTATGAGGTCCCCTGCGGGGTACCTCACCTCAGCCATTGA531070.11139096178346354No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA530220.11121267583713641No Hit
GGTTGGTGCGCGGACACGCACTGCCTGCGTAACTAGAGGGAGCTGACGGA527350.1106106985830672No Hit
GCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAA511310.10724633790178835No Hit
GCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAAC486260.10199214618944202No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG481200.10093082043836528No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)