FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005296110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005296110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50525478
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2485000.49183107184062663No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC2316060.4583944757534011No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG1054740.2087540864036952No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT958890.18978345934698532No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC866400.1714778433169895No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAGTCAATCTCGTAT809630.1602419278447994TruSeq Adapter, Index 18 (97% over 38bp)
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT727570.14400061687689525No Hit
CAGCAGATCATTTCATATTGCTTCCGTGGAGTGTGGCGAGTCAGCTAAAT722820.1430604971218679No Hit
GTTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCCTAAGATAGAG645670.1277909731007394No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA587620.11630172009456298No Hit
GGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTA578420.11448085656903632No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA578230.11444325177883523No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA532600.10541216453211981No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)