FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005296564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005296564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18625396
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2423801.3013414587265688Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT345000.0110.415578
GAGCGTC347850.0109.179539
ATCGGAA357850.0107.632972
TCGGAAG359300.0107.018263
GATCGGA366100.0105.36141
AAGAGCG365150.0104.763827
CGGAAGA368300.0104.18784
GAAGAGC513000.074.954116
GGAAGAG629100.061.567855
CGCCGTA277800.026.34901245-49
GCCGTAT283900.025.74736845-49
CCGTATC283200.025.71441545-49
CGTATCA284000.025.53551945-49
TCGTGTA312750.024.07470110-14
TCGGTGG315650.023.8650635-39
CGTGTAG317950.023.84375215-19
TCTCGGT318100.023.56809835-39
GTGGTCG316950.023.34937940-44
GTCGTGT324700.023.31725510-14
TCGCCGT315450.023.13114445-49
Produced by FastQC (version 0.11.5)