FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005296567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005296567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31858348
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT43500.033.72316445
TCTCGTA45650.032.1351944
TATCTCG46150.031.54326442
ATCTCGT49450.029.71125243
CGTCTGA51400.029.63486917
ACACGTC52100.029.19342414
ACGTCTG60350.025.61279116
CACGTCT61950.024.80596715
GCACACG70050.021.80889512
CACACGT78050.019.8043213
GGATATC81050.018.40497639
CGGAAGA83300.018.367715
TCGGAAG86650.017.6588944
GTCACTA88150.017.30566430
ATCGGAA94500.016.4541783
TCACTAC99450.015.70137131
GATCGGA103200.015.4377092
CGACGCT5550.014.59701251
GATATCT110800.013.68655740
AGATCGG120250.013.3993091
Produced by FastQC (version 0.11.5)