FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005296570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005296570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36264665
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT37500.031.1393245
TCTCGTA40400.028.79288744
ATCTCGT49250.023.89304543
CGTCTGA67800.017.8869517
ACACGTC74700.016.11428314
ACGTCTG77400.015.55213516
CACGTCT84900.014.07226815
GGTATGA91750.013.29126437
CGACGCT10500.013.073451
GTATGAT98200.012.23505138
TATGATC100450.012.11778739
GCACACG104750.011.6847912
TGGTATG107300.011.59573936
CACACGT106100.011.4937513
GATCTCG112000.011.22974342
CTTGGTA108900.011.17744234
ACTTGGT123400.09.95529533
TTGGTAT125900.09.88262835
TCCGATC14400.09.68735510
GTCACTT137500.09.24540530
Produced by FastQC (version 0.11.5)