FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005296754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005296754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43568732
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA701110.16092045093256327No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG692850.1590245958959742No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG550370.12632224412682014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG235500.015.85534645
GGGAGGT373700.015.0349833
GGAGGTC373950.014.7660184
GAGGTCA383150.014.3409525
ATAGGGG267000.013.94833838
GGCATAG266150.013.94558235
GTCACTC397950.013.7270678
GAGCTTA274100.013.69729944
GGTCACT404600.013.4348967
ACTCTCC409200.013.32311111
TGGCATA285300.013.05759634
ACCCGGG101250.013.0334092
TCACTCT427350.012.9412919
CACTCTC434150.012.484488510
CACCCGG113050.012.011611
CATAGGG326750.011.50182837
TGGGAGG357200.011.28488352
GTTGTCG25550.011.27082426
GCATAGG340800.011.03472436
AGCATAG154550.010.91708145
Produced by FastQC (version 0.11.5)