FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005296805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005296805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45732858
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT751490.16432167873698164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT158200.023.49784545
ACCGCGT173200.021.2801326
AGACCGC174300.021.1587454
GACCGCG178550.020.6299025
CAGACCG181550.020.400553
CCGCGTT186650.019.7572737
CGCGTTC190150.019.3459428
AGAACGA199950.018.60212744
TTCGACG17800.017.571276
TACGGAG212050.017.49642834
AATACGG211050.017.11026432
TCAGACC227750.016.8846662
ATACGGA220400.016.87439733
AAGAACG221500.016.87322243
CAATACG229700.015.85816331
GCGTTCT239250.015.5449519
ACGGAGA244500.015.18350735
CTCAGAC281150.014.8237881
CGGAGAG263050.014.1042336
AACGCTA68700.013.85321723
Produced by FastQC (version 0.11.5)