FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005296811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005296811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44133159
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1766450.4002546022141764No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1039120.2354510811247389No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG770900.1746759165823593No Hit
GGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGC504890.11440150930505563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG24400.020.099086
CAGACCG286050.019.7947853
AGACCGC285400.019.7373984
GACCGCG297000.018.951365
ACCGCGT298450.018.8668256
CCGGCTT576900.018.5694222
TTCACGC589850.018.3219187
CGGTATA35900.018.29780220
GCCGGCT544750.018.0031071
TCACGCT611550.017.6386858
TCGACGA28700.017.4012577
CGTTACG24450.017.29774521
TCAGACC341600.017.2738822
CCGCGTT328750.017.1621347
CGGCTTC648900.017.122643
CTACACG229300.017.01559345
CGCGTTC336950.016.664368
ACGCTCA653000.016.4604810
CTCAGAC383100.016.3724291
GTTCGAC28750.016.2755265
Produced by FastQC (version 0.11.5)