FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005296825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005296825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43439534
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG657520.1513644230161401No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT485210.11169779123321166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC160300.018.356474
ACCGCGT165500.017.507856
CAGACCG170600.017.4196243
GACCGCG167150.017.3081055
TCGTTAC27350.017.1132144
ACGTCCG22900.017.00086832
CGTTACG31900.016.99623921
TCACGCT262050.015.8816468
CCGCGTT182850.015.858647
TTCGACG17350.015.8188666
AACGCTA250000.015.76699923
CGCGTTC184950.015.629928
ACGCTAC253000.015.57114624
GCCGGCT255350.015.4717491
TTCACGC272800.015.3135377
CCGGCTT289600.015.1012432
CGCTACC260450.015.065968525
AAAACGC266950.014.85760621
TCAGACC211900.014.7039422
GAAAACG271300.014.52769720
Produced by FastQC (version 0.11.5)