FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005296829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005296829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42757412
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT789490.18464400979179937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC191750.020.906694
CAGACCG197000.020.62363
GACCGCG199600.020.1408125
ACCGCGT199850.020.104366
TCAGACC232300.017.6542972
CCGCGTT237350.016.8896947
CGCGTTC244750.016.3606538
TCACGCT192100.015.70388
CTCAGAC281700.015.4967251
TTCACGC198900.015.2008487
CCGGCTT226100.014.6758912
ACGCTCA215400.014.05732410
GCCGGCT239350.013.7401811
GCGTTCT296400.013.5931699
AAAAACG126350.013.22926421
CACGCTC232900.013.145959
CGGCTTC262900.012.9553243
TTCGACG13850.012.6694516
GTCCGTT32300.012.2739171
CGTTCTC337450.012.16625110
Produced by FastQC (version 0.11.5)