FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005296835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005296835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43364539
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT742920.17131970433261148No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG560240.12919311790677632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC204000.019.1106914
GCCGGCT247100.018.2045751
CAGACCG218800.017.9825153
GACCGCG219650.017.595435
ACCGCGT228350.016.9940176
CCGGCTT285300.016.9844672
TTCGACG16500.016.769856
TTCACGC286000.016.6439957
TCACGCT294600.016.1733958
TCAGACC263150.015.5758932
CTCAGAC290950.015.0815431
CGGCTTC337650.015.0040883
ACGCTCA320550.014.86408410
CACGCTC328750.014.5343879
CCGCGTT268300.014.4300627
GAAAACG240050.014.25415520
CGTTACG21450.013.94902521
CGCGTTC279600.013.9112398
TCGACGA21600.012.9144517
AACGCTA294300.012.52938423
Produced by FastQC (version 0.11.5)