FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005296845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005296845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45107726
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2146150.47578323943884915No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG716960.15894394676424167No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA665670.14757338909081782No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG640450.141982329146896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT474800.022.56859445
AGACCGC490700.022.0293254
ACCGCGT501750.021.4634276
GACCGCG505050.021.309825
CAGACCG510950.021.213653
TCAGACC548000.020.1448542
CCGCGTT535400.020.1166657
CGCGTTC540850.019.9139338
CTCAGAC600600.019.172641
AGAACGA566500.018.94300744
GCGTTCT576550.018.723589
CGTTCTC641050.017.23628610
AAGAACG631300.016.99427843
AATACGG642000.016.6045532
TACGGAG644550.016.4934834
ATACGGA651000.016.406133
CCTCTCA670000.016.30755219
ACTCCCC673550.016.22088225
CAATACG664400.016.14965831
ACGGAGA667500.015.92991235
Produced by FastQC (version 0.11.5)