FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005296903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005296903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42963406
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1159250.2698226486047219No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG747840.17406441193233144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC278100.022.7047214
CAGACCG285600.022.2423783
ACCGCGT287950.021.9124286
GACCGCG290850.021.6320725
TCAGACC323000.020.467832
CCGCGTT317600.019.8667627
CGCGTTC324000.019.4812748
CTCAGAC365000.018.810921
GCGTTCT361850.017.5181059
TCACGCT287500.016.7514938
TTCACGC291750.016.6693847
CCGGCTT309250.016.1479722
GCCGGCT281050.015.9711511
AACGCTA290050.015.80876823
ACGCTAC290750.015.7711324
CGTTCTC428550.015.46868410
ACGCTCA318350.015.38254910
CGCTACC298600.015.30377125
CACGCTC326250.014.9618039
AAAACGC309350.014.90900821
Produced by FastQC (version 0.11.5)