FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005296937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005296937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46842863
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG585580.12500943847091497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCTT214350.017.4426922
GCCGGCT202250.017.1345121
AACGCTA206950.016.95055823
TTCACGC212000.016.9250837
ACGCTAC209000.016.75228524
TCACGCT219900.016.4091138
CGCTACC215900.016.16490625
AAAACGC223500.015.74447721
ACCGCGT109750.015.168176
CGTTACG26700.015.16806921
GACCGCG108200.015.1359655
ACGCTCA243000.014.97884210
CGGCTTC264800.014.9520473
GAAAACG232900.014.92511920
AAACGCT240250.014.70299322
AGACCGC113150.014.6527424
TCGTTAC20850.014.57254344
CACGCTC253700.014.24955759
CCGCGTT121700.013.6972577
GTTTTCG253650.013.66459645
Produced by FastQC (version 0.11.5)