FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44042672
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA14500.019.23072819
AGACCGC116500.018.8900664
CAGACCG123650.017.9328443
GACCGCG129150.017.0560055
ACGAACG16350.016.7695518
ACCGCGT131200.016.7412326
CGCTACC163650.016.2079925
AACGCTA185900.016.13169923
ACGCTAC188850.015.91857524
CGTTACG22450.015.53215821
CGGCAGT449100.014.9960740
AGTTGCA440200.014.98348844
AAAACGC202950.014.98267921
TCAGACC157500.014.7680582
GAAAACG177200.014.6540720
ACGGCAG467800.014.38027439
AAACGCT216300.014.07605622
CAGTTGC472700.013.89614543
CTACACG54700.013.85597545
CGTGGTT219250.013.72418441
Produced by FastQC (version 0.11.5)