FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47885975
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG758480.1583929323773819No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT689130.14391061265850805No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG479430.10011908497216564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA244300.017.58052323
ACGCTAC246600.017.41675224
AGACCGC192100.017.3783574
CAGACCG194900.017.3133683
CGCTACC250200.017.16616825
TTCACGC260150.017.0783317
TCACGCT263250.016.8686738
GACCGCG200750.016.5399045
CCGGCTT284600.016.441092
AAAACGC263300.016.43138321
ACCGCGT202550.016.3929186
GCCGGCT267750.016.3058381
GAAAACG273450.015.86258120
AAACGCT275750.015.70601122
CGTGGTT278800.015.41035441
ACGCTCA279400.015.3944310
GTTTTCG278650.015.31491645
CTACACG77650.014.6960945
CACGCTC289500.014.6397769
CGGCTTC336500.014.5805113
Produced by FastQC (version 0.11.5)