FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45673306
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT569860.12476872158104779No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG468230.10251721213261857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG16000.017.293216
AGACCGC176850.016.1034564
AACGCTA226750.016.0158123
ACGCTAC229500.015.85192324
TTCACGC238950.015.76878457
CCGGCTT252050.015.61858752
ACCGCGT182150.015.5484476
CAGACCG187950.015.2960383
CGCTACC189300.015.2013525
GACCGCG186650.015.1615325
GCCGGCT239800.014.9961321
TCACGCT253000.014.8753048
AAAACGC245500.014.86563521
GAAAACG215700.014.86422220
CCGCGTT193050.014.6822017
CGCGTTC197550.014.3591438
CGTTACG17750.014.32399721
AAACGCT255450.014.32194722
CTACACG78650.014.19402145
GTTTTCG255750.014.14238845
Produced by FastQC (version 0.11.5)