FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45673306
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG537100.117596041766716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG187750.014.1882545
GGGAGGT330150.011.8051093
GAGCTTA225700.011.74267544
GGCATAG226300.011.73188135
ATAGGGG230900.011.55163638
GGAGGTC345750.011.0317994
TGGCATA242350.010.98279434
ACTCTCC371350.010.09585211
GTTGTCG23750.010.03748726
GGTCACT371400.010.0155857
GTCACTC376950.09.9516498
CATAGGG276550.09.72613437
TAGCGTA8650.09.6198537
GCATAGG285250.09.46204136
CACTCTC405450.09.21885410
TGGGAGG320150.09.1040412
CGTGGCG146650.08.986661
TGCCCGT134900.08.921144544
TGTCCAA431100.08.73293326
AGCATAG118350.08.70240345
Produced by FastQC (version 0.11.5)