FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45388745
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG743610.16383136392072528No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT466180.10270828153543352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC124550.018.7444444
CAGACCG132250.017.9762173
GACCGCG133250.017.5881215
ACCGCGT138500.016.9449586
TTCGACG17000.015.4712956
ACGCTAC115400.015.36801324
AACGCTA115550.015.26982123
CGGCAGT267600.014.78859240
CGCTACC121000.014.65678325
TTCGGCG73600.014.54038244
GTCCGTT32650.014.3972551
GACGGCA274450.014.16136938
ACGGCAG276250.014.06866139
TGACGGC281750.013.89038737
CCGCGTT171800.013.5950017
AAAACGC133150.013.59332221
TCAGACC183700.013.4557382
TCGGCGC78600.013.41503245
CGCGTTC178400.013.1550518
AGTTGCA301550.013.05489844
Produced by FastQC (version 0.11.5)