FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44990709
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT672150.149397512273034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC183400.019.6101634
ACCGCGT186400.019.211476
CAGACCG191650.018.9655133
GACCGCG190900.018.7808175
CCGCGTT199850.017.9072197
CGCGTTC201900.017.7253978
GAACGAT199550.016.88830245
TCAGACC231750.015.8682882
CCGGCTT194800.015.4374132
GCGTTCT234900.015.3021279
ACGCTAC175350.015.21577124
AACGCTA175900.015.20635623
GTCCGTT35300.015.0371831
TCACGCT191100.014.8099738
TCGTTAC17450.014.68759844
CGCTACC182400.014.64003325
TTCACGC193800.014.5804437
AGAACGA236350.014.35406744
GCCGGCT194400.014.3005061
CTCAGAC284850.014.2445751
Produced by FastQC (version 0.11.5)