FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45864686
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG154850.013.11376745
GGCATAG173450.011.68131635
GGGAGGT268050.011.5791373
ATAGGGG177650.011.45788238
GAGCTTA181400.011.20369344
GGAGGTC276350.010.8732634
TGGCATA201350.010.12978334
AGCGCTC64650.010.0540899
CATAGGG212950.09.61117937
ACGTCTC63950.09.60209121
CGGGACG62800.09.56307817
CGCTCTC71550.09.273411
GCATAGG222400.09.26346836
ACTCTCC324200.09.15078511
GGTCACT326850.08.9318037
TAGGGGA334600.08.75198439
GTAACGA45800.08.594460518
AGCATAG103650.08.58898245
GTCACTC351050.08.5787598
TCTCGGG72900.08.51567614
Produced by FastQC (version 0.11.5)