FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46413411
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTCCAGCAC667290.14377094585873038No Hit
CCTGCTGCCACCTGCTCTTGTCCACGGTGAGCTTGCTGTAGAGGAAGAAGG641580.13823159862135537No Hit
CCACGGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTCCAGCACGG607320.13085011140422323No Hit
CTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTC559570.12056213666347428No Hit
CCCTGCTGCCACCTGCTCTTGTCCACGGTGAGCTTGCTGTAGAGGAAGAAG517680.11153672803750622No Hit
CTGGGCTTGTGATTCACGTTGCAGATGTAGGTCTGGGTGCCCAAGCTGCTG479500.10331065734427493No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG478800.10315983886640007No Hit
CTCTTGTCCACGGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTCC467860.10080276151218448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT12750.018.7027931
AACGCTA168050.017.39705823
AGACCGC116700.017.2641414
ACGCTAC172800.016.98404124
CGCTACC173150.016.88487825
TTCGACG12750.016.7541076
TTCACGC181550.016.6584227
CCGGCTT203550.016.459742
ACCGCGT123400.016.071686
GAACGAT130000.016.00859845
AAAACGC183700.016.00012621
CAGACCG130550.015.4842663
AAACGCT191700.015.32079422
GTTATAA132700.015.30829440
ACGCTCA203450.015.21892810
CGGCTTC225050.015.1470493
GAAAACG193150.015.112473520
GTCCGTT22800.015.08480551
TCACGCT205250.014.8225358
GTTTTCG194000.014.82122345
Produced by FastQC (version 0.11.5)