FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46413411
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGG560220.1207021823929295No Hit
GGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGAC485530.10460985080368258No Hit
GGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCG484670.10442455953086491No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCTC70850.013.8406359
AGCTTAG110100.012.5527545
GCAGCGC87400.011.8631197
CAGCGCT89700.011.7845998
CGCTCTC88200.011.32218711
GGCATAG127050.010.96863835
ATAGGGG130750.010.80257638
GAGCTTA130450.010.57056744
GGGAGGT201750.010.2672823
GTCAGGA183850.010.2270891
TGGCATA139350.09.96815934
CATAGGG142600.09.73139437
GGAGGTC203900.09.62955954
CGTCAGT160900.09.4632871
TCAGTTC145950.09.4617993
CGGGACG48700.09.37576917
GCATAGG151800.09.28977436
ATAACGC102400.09.09305439
TAACGCC107900.08.8676140
GGTTCTT184500.08.8501336
Produced by FastQC (version 0.11.5)