FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46722728
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1614950.34564548542627904No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA647630.13861134135832137No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG580000.12413658723009495No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG513680.10994221056612961No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG512930.10978168911712517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC414100.019.184134
CAGACCG417900.019.0850163
GACCGCG422750.018.7596865
ACCGCGT424800.018.6620246
TCAGACC460750.017.4711482
CCGCGTT473850.016.7255027
CTCAGAC513850.016.5146981
CGCGTTC486950.016.2478478
GCGTTCT541050.014.706159
CGTTACG25850.014.70516221
CTACACG65650.013.76886945
AACGCTA213400.013.72491923
ACGCTAC215050.013.54682724
CGTTCTC593750.013.5410710
GAACGAT588500.013.411245
CGCTACC221700.013.13025825
GTTTTCG224850.013.01042245
AAAACGC232900.012.62262721
TTCACGC252000.012.3926067
TCACGCT252550.012.2854968
Produced by FastQC (version 0.11.5)