FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45476950
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT622050.13678357937372668No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG483220.10625602640458517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC161000.018.5836034
GACCGCG163800.018.2933835
ACCGCGT166250.018.0236176
CAGACCG167600.018.012743
CCGCGTT187550.015.976687
CGCGTTC193800.015.4729848
AACGCTA132350.015.3355823
ACGTTCG15950.015.2207656
ACGCTAC135650.015.04588824
TCAGACC205700.014.9823842
CGCTACC142750.014.20285925
AAAACGC150300.013.647614521
TACGGTA52700.013.22360444
CGTTACG17050.013.1915721
CTCAGAC255000.013.1790191
GTCCGTT35400.013.0811531
GAAAACG157400.012.9736420
CTACACG49850.012.67184645
GCGTTCT240550.012.5871419
CCTTACG56500.012.579926541
Produced by FastQC (version 0.11.5)