FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46437754
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG913040.19661588284394632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT237600.020.5439951
CCGGCTT271400.019.8443742
TTCGACG18350.019.7384436
TTCACGC272900.019.1087827
TCACGCT275400.018.9353188
AGACCGC121750.017.5355874
AACGCTA299400.016.95370523
ACGCTAC300100.016.94409424
CAGACCG128000.016.9078433
CGGCTTC339650.016.4992873
CGCTACC307650.016.48449725
ACGCTCA323250.016.2158810
AAAACGC319950.015.87890621
GTCCGTT34050.015.8622911
GAAAACG325700.015.667335520
TCGACGA24450.015.5500187
AAACGCT329350.015.48713822
CGTTACG31000.015.38696321
CACGCTC341850.015.2940899
CGGTATA35200.015.08478720
Produced by FastQC (version 0.11.5)