FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47309196
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2101890.4442878293683114No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA728950.15408209431417944No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG667900.14117762643863152No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG558130.11797494931006648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT505150.021.561896
AGACCGC510800.021.5083684
CAGACCG513400.021.4432683
GACCGCG509700.021.351735
GAACGAT508550.021.2780945
CCGCGTT524050.020.7919167
CGCGTTC528150.020.6730428
TCAGACC556900.020.212662
CTCAGAC582600.019.9338761
TTCGACG37350.019.6380836
GCGTTCT569150.019.2391389
GTTCGAC39000.018.4033835
TCGACGA40300.018.3114017
CGTTCTC610300.018.2847610
AGAACGA629500.017.24698344
CGGTATA52000.017.21988520
AATACGG653550.016.55479632
CCTCTCA674500.016.41094819
TACGGAG654600.016.4079434
ATACGGA661000.016.37161333
Produced by FastQC (version 0.11.5)