FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42853067
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1700410.3968000703426898No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG773140.18041649154306738No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA596330.13915690095180352No Hit
CTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTG575890.13438711399583136No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCCC547090.1276664748406456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT388350.022.97291645
AGACCGC406900.021.9154284
GACCGCG405250.021.8270245
CAGACCG409450.021.800923
ACCGCGT406850.021.7688316
CCGCGTT425650.020.8179237
TCAGACC444450.020.6105182
CGCGTTC431200.020.5499748
TTCACGC343150.018.8673467
AGAACGA474250.018.85060344
CTCAGAC505150.018.8373641
TCACGCT342800.018.834118
GCCGGCT328750.018.7964841
GCGTTCT477950.018.6716989
CCGGCTT357550.018.591942
AATACGG488950.018.10230432
ATACGGA495100.017.918633
TACGGAG496700.017.77028334
TTCGACG17500.017.739656
CAATACG510700.017.48478731
Produced by FastQC (version 0.11.5)