FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42853067
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG560400.1307724368946568No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG552420.1289102597954074No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA491320.11465223714325978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCACT308250.013.2575837
GTTGTCG26350.013.06423826
GGGAGGT331800.013.0428773
GTCACTC323550.012.6375738
AGCTTAG234750.012.51919845
GAGGTCA342250.012.44726855
GGAGGTC340700.012.4048524
GGCATAG255100.011.54460935
TGCCCGT182750.011.42212644
ATAGGGG264800.011.23086238
TGGCATA268000.011.098330534
TCACTCT381450.010.9908649
ACTCTCC380300.010.91789911
GAGCTTA269050.010.91518744
CACTCTC392200.010.563457510
TGGGAGG297950.010.4588512
ATATAGC35800.010.3145216
CTGGGAG343150.010.0259971
AGGGATC159950.09.7552932
TGTCGTT35000.09.64028528
Produced by FastQC (version 0.11.5)