FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46250407
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA853340.18450432230790964No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA681690.147391135390441No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG680160.1470603274907397No Hit
GTTTAAACAGTTCAGTGATCTTTAGTGCATTGTTTATGTGTGGGTTTCTCT613110.13256315776853597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGTC224250.011.7197434
AACTCTA108400.011.57688845
GGGAGGT236150.010.9762233
TAATCGG124900.010.9714134
AATCGGT128350.010.8520765
ATAATCG129800.010.7647853
GTAACGA124650.010.67110918
GGTAACG126650.010.64476317
ATCGGTT131050.010.525896
GTGTCGA129500.010.44308727
GAGGTCA252300.010.363515
GGGTTGA131450.010.29974744
CGTAACG20550.010.17824842
GGTCACT249100.09.991317
TCTATAG526900.09.8729743
TACGGTT48300.09.87269635
TGTCGAG142700.09.74475128
TTACGGT50450.09.67529934
TTCTATA558750.09.575682
CATGCAT78650.09.5278329
Produced by FastQC (version 0.11.5)