FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44423586
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG123200.013.27424838
GGCATAG122550.012.99624635
GTCACTC191250.012.7870558
GGTCACT191800.012.59797
GGGAGGT227200.011.9289233
GGCGTAG69400.011.79885535
CATAGGG137250.011.76786937
GCGTAGG69350.011.74248736
CGTAGGG69850.011.69030837
ACTCTCC214450.011.54065811
AGCTTAG139500.011.48207545
GGAGGTC226000.011.4245214
AGGTCAC230250.011.1972736
CGTGGCG93900.011.082441
TGGCATA146050.011.02832134
GAGCTTA149700.010.92514544
TAGGGGA223550.010.82763639
GCATAGG148250.010.77361936
GTTGTCG21600.010.62539626
CACTCTC233000.010.5832410
Produced by FastQC (version 0.11.5)