FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43096235
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGATC124750.011.0702622
GACGCAA54250.010.57364344
ACGCAAG59150.09.8875645
CTTGACG166500.09.71549442
CGTGGCG51600.09.6488931
ACTAACC104550.09.61840338
AACTAAC108850.09.40368237
TTGACGT174100.09.35559543
CGACCCG55200.09.254796
CAGGGAT176650.09.0930761
GTAGCGT70350.08.95291645
GGGATCA169800.08.7873043
CGATAAG68650.08.74996617
TCGATAA70600.08.47654916
CACCGAA242050.08.44752345
GAGACGC76150.08.42025442
TAAGTCG69150.08.39440320
GCGTCGT17900.08.29590611
GGTAGCG78000.08.27697244
CTAACCA123850.08.24696139
Produced by FastQC (version 0.11.5)